A plasmid containing a normal human beta-globin allele is digested with restriction enzymes. The results are shown in lane 2 of the electrophoresis gel. A second plasmid, contain the beta-globin allele that results in sickle-cell disease, is digested in the same way and placed in lane 3. The standard in lane 1 contains DNA fragments in lengths of 100 base pairs (bp), 200 bp, and so on. Which of these describes the results shown on the gel?